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  Indian J Med Microbiol
 

Figure 3 Agarose gel electrophoresis for the products of the nested PCR targeting the Cryptosporidium oocyst wall protein (COWP) gene of Cryptosporidium at 553 bp and restriction-fragment length polymorphism products after digestion with RsaI endonuclease. Lane 1: 100 bp DNA marker ladder. Lanes 2, 3, and 4: Cryptosporidium parvum genotype 2 digestion products at 34, 106, and 410 bp (34 band is very small, faint, and difficult to see). Lanes 5 and 6: Cryptosporidium hominis genotype 1 digestion products at 34, 106, 125, and 285 bp. Lanes 7 and 8: product of nested PCR targeting the COWP gene at 553 bp.

Figure 3 Agarose gel electrophoresis for the products of the nested PCR targeting the <i>Cryptosporidium</i> oocyst wall protein (COWP) gene of <i>Cryptosporidium</i> at 553 bp and restriction-fragment length polymorphism products after digestion with <i>Rsa</i>I endonuclease. Lane 1: 100 bp DNA marker ladder. Lanes 2, 3, and 4: <i>Cryptosporidium parvum</i> genotype 2 digestion products at 34, 106, and 410 bp (34 band is very small, faint, and difficult to see). Lanes 5 and 6: <i>Cryptosporidium hominis</i> genotype 1 digestion products at 34, 106, 125, and 285 bp. Lanes 7 and 8: product of nested PCR targeting the COWP gene at 553 bp.