Year : 2014 | Volume
: 7 | Issue : 2 | Page : 104--109
The epidemiology of Giardia intestinalis assemblages A and B among Egyptian children with diarrhea: A PCR-RFLP-based approach
Nora L El-Tantawy, Amira I Taman
Department of Parasitology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
The protozoan parasite Giardia intestinalis is a common childhood infection in developing countries that causes diarrheal illness. The majority of G. intestinalis isolates from humans are grouped into two distinct genetic assemblages A and B. The molecular epidemiological studies on G. intestinalis assemblages in humans are limited in Egypt.
This study was conducted to estimate the detection rate of G. intestinalis infection among a cohort of children suffering from diarrhea in the Dakahalia governorate, Egypt, and to correlate between clinical giardiasis and Giardia spp. assemblages in positive stool samples by PCR-restriction fragment length polymorphism (PCR-RFLP).
Participants and methods
A total of 311 diarrheal stool samples were examined microscopically for Giardia spp. infection. DNA samples were isolated from the stools of 103 (33.12%) positive samples with G. intestinalis, amplified with PCR, and digested with the XhoI enzyme for RFLP.
Of the 103 samples, 64 (62.14%) were found to be assemblage B, whereas 32 samples (31.07%) belonged to assemblage A. Mixed genotype A and B was present in three samples (2.91%), and four samples (3.88%) were of undetermined Giardia spp. assemblage. The detection rate of assemblage B was higher in samples from children with persistent diarrhea, whereas assemblage A detection rate was higher in samples from acute diarrhea.
G. intestinalis causing diarrhea in children in the Dakahalia governorate, Egypt, predominantly belongs to assemblage B, indicating that human-to-human method of infection is more common than zoonotic method.
Nora L El-Tantawy
Department of Medical Parasitology, Faculty of Medicine, Mansoura University, 35516, Mansoura
|How to cite this article:|
El-Tantawy NL, Taman AI. The epidemiology of Giardia intestinalis assemblages A and B among Egyptian children with diarrhea: A PCR-RFLP-based approach.Parasitol United J 2014;7:104-109
|How to cite this URL:|
El-Tantawy NL, Taman AI. The epidemiology of Giardia intestinalis assemblages A and B among Egyptian children with diarrhea: A PCR-RFLP-based approach. Parasitol United J [serial online] 2014 [cited 2020 Sep 29 ];7:104-109
Available from: http://www.new.puj.eg.net/article.asp?issn=1687-7942;year=2014;volume=7;issue=2;spage=104;epage=109;aulast=El-Tantawy;type=0