|Year : 2016 | Volume
| Issue : 1 | Page : 1-6
Applications of nanomedicine in parasitic diseases
Sherif M Abaza PhD
Department of Medical Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
|Date of Submission||24-Mar-2016|
|Date of Acceptance||26-Apr-2016|
|Date of Web Publication||25-Oct-2016|
Sherif M Abaza
Department of Medical Parasitology, Faculty of Medicine, Suez Canal University, Ismailia
Source of Support: None, Conflict of Interest: None
Nanomedicine is defined as application of nanotechnology for treatment, monitoring, prevention, and control of biological diseases. To apply nanomedicine, the precise targets (cells and/or receptors) specific to the clinical disease should be identified and the suitable nanoparticles for delivery system to minimize the side effects of the original drug should be selected. One of these precise targets are macrophages, endothelial, dendritic as well as tumor cells. The main aim of the present review is to throw light on possible nanotechnology applications in parasitic diseases focusing on three main aspects: diagnosis, treatment, and vaccination.
Keywords: adjuvant, diagnosis, drug delivery system, gene therapy, macrophage, nanoparticles, parasitic diseases, treatment, vaccine
|How to cite this article:|
Abaza SM. Applications of nanomedicine in parasitic diseases. Parasitol United J 2016;9:1-6
Reduction of certain materials from macro to nano scale is associated with changes in some of their properties. For example, gold becomes liquid at room temperature, silicon turns into a conductor instead of an insulator, and aluminum becomes combustible. The synthesis and properties of semiconductor nanocrystals were studied, leading to the discovery of a number of metal and metal oxide nanoparticles (NPs) . This was followed by the use of NPs in nanomedicine for the diagnosis and monitoring as well as the treatment and control of biological diseases. The use of NPs is increasing as they have defined chemical, optical, and mechanical properties . In the new century, there has been a strong focus on applications in the diagnosis and treatment of cancer, e.g. formulation of therapeutic agents in polymeric, biocompatible, and submicron-sized composites. A recent review article discussed the principles of nanotechnology and applications of nanomaterials in medicine and techniques as well as the instruments used to investigate the physicochemical characteristics (size, shape, surface properties, composition, purity, and stability) of the commonly used nanomaterials .
Cytotoxicity associated with nanotechnology raised certain concerns in terms of the individual physicochemical properties (size, charge, concentration, and outer coating bioactivity) and environmental conditions (oxidative, photolytic, and mechanical stability). For example, some NPs were found to be cytotoxic only after oxidative and/or photolytic degradation of their core coatings. Few in vitro and in vivo studies have suggested that NPs can affect cell growth and viability in a dose-dependent manner. The most important factor of NPs toxicity is their stability, both in vivo and during synthesis and storage .
Nanomaterials include silver, gold, platinum, and magnetic NPs, quantum dots, liposomes, nanospheres, carbon nanorods, nanotubes, and others. Silver ,,,,,,,,, and gold ,,,,,,,,, NPs were the commonest elements used, and constituted the majority of publications in the last 10 years. However, other elements have been used such as calcium , iron oxide , and silica . Chitosan (CS) is another NP formula, formed of the natural polysaccharide Arabic gum, that attracted significant scientific interest during the last two decades because of several properties: 1) it is nontoxic, 2) it has various molecular weights, 3) it forms complexes with DNA that effectively protects it from degradation, 4) it enhances the penetration of large molecules across the mucosal surface, and 5) it is taken up by Payer’s patches. Therefore, it has been used in drug delivery, tissue engineering, and gene delivery ,,,,,,,,,,. Liposomes and microspheres are considered the most extensively studied carriers for drug delivery systems. Liposomes are nano-sized vesicles formed of layers of natural phospholipids and cholesterol surrounding an aqueous space. According to the size and number of layers, they are classified into three types: small unilamellar, large unilamellar, and multilamellar liposomes. According to the physicochemical characteristics of the drug, it is either entrapped in the aqueous space or inserted into the liposome layers ,,,,,. Nanospheres consist of synthetic or natural polymers (collagen or albumin), and the required drug is conjugated or encapsulated within the polymeric matrix ,,,. Other chemical components were also reported; e.g. self-assembling polypeptide (SAP), solid lipid (SL), polyamidoamine (PAMAM), polylactide-co-glycolide (PLGA), polyethylimine, γ polyglutamic acid (γ-PGA), and archaeosome vesicles (ARC) which are made from lipids extracted from Archaea. Their NPs are helpful to carry the antigen to dendritic cells (DCs) due to two important characteristics: its tiny size which allows it to cross the skin’s extracellular matrix, and its coating which mimics a bacteria cell wall causing the complement system to be activated ,,,,,.
| Applications in parasitic diseases|| |
Regarding the diagnosis of malaria, recombinant heat shock protein 70 (HSP70) of P. falciparum conjugated with gold NPs and functionalized with anti-HSP70 monoclonal antibodies proved to be very sensitive in the detection of malaria antigen . Polystyrene NPs conjugated with polyclonal IgG antibodies specific to P. falciparum showed sensitive results . Specific DNA aptamers (oligonucleotide molecules selected from pools of random-sequence oligonucleotides in the P. falciparum DNA library to bind a wide range of relevant proteins) were conjugated to gold NPs to achieve rapid and early diagnosis of malignant malaria ,. The Wondfo rapid diagnostic test linked P. falciparum biomarkers (histidine-rich protein-2 and pan-Plasmodium lactic dehydrogenase, Pf-HRP2/PAN-pLDH) with gold NPs in an immunochromatographic (ICT) assay to achieve better diagnosis of malaria in clinical samples . Screening of blood films was successfully achieved by the detection of hemozoin or β-hematin (unique components of Plasmodium spp.-infected red blood cells) using surface-enhanced resonance Raman spectroscopy (SERRS) and atomic force microscopy (AFM). NPs were modified (tuning the core and shell with a silver shell) and used in SERRS allowing magnetic field. This tuning causes aggregation of β-hematin, and early diagnosis of malaria . While AFM was successfully used to generate transient vapor nanobubbles around the hemozoin in response to a short infrared picosecond laser pulse .
In the diagnosis of other parasitic diseases, the same concept of NPs conjugation with the parasite biomarkers was used. In toxoplasmosis, the use of specific agglutination of antigen-coated gold NPs in the detection of the corresponding antibody gave satisfactory agreement with ELISA results . An immunomagnetic bead-ELISA technique utilizing T. gondii IgG polyclonal antibodies coated with magnetic NPs to capture circulating surface antigen 1 gave better results than sandwich ELISA . In cryptosporidiosis, HSP70 was also conjugated with gold NPs to target HSP70 mRNA from C. parvum oocysts . An assay that used probes of oligonucleotide-functionalized gold NPs (complementary to the 18s rRNA sequence on C. parvum) proved its ability to detect the nucleic acids of C. parvum oocysts in stool samples . For amebiasis, fluorescent silica NPs were synthesized and conjugated with monoclonal anti-E. histolytica IgG1 for diagnosis of amebiasis. It showed high sensitivity results without cross reaction with other protozoa (E. dispar, E. moshkovskii, G. lamblia and Blastocystis spp.) . Synthesized iron oxide NPs were functionalized with CS and used to capture and remove Entamoeba spp. cysts after application of an external magnetic field. The investigators claimed that the synthesized NPs were well dispersed and suitable for water treatment. They suggested that cyst wall components (lectins and chitin) might interact with CS NPs, and recommended further studies to validate the use of NPs in water treatment, with a special emphasis on possible NPs toxicity . In leishmaniasis, gold NPs conjugated with four oligonucleotide probes, targeting DNA of Leishmania kinetoplastid, were used . Recently, gold NPs were also conjugated with labeled Leishmania spp. primers and magnetic beads for isothermal amplification of Leishmania DNA in blood samples of infected dogs. It was found that NPs have electrocatalytic activity for the rapid detection of the amplified DNA. This approach was found to be more sensitive and less expensive than the traditional PCR methods used in the diagnosis of visceral leishmaniasis (VL) .
When specific antibodies are coated with laser-irradiated gold NPs, they selectively attach to the parasite, and the heat produced from the irradiated laser will kill the parasite . Besides, the macrophage is a valid pharmaceutical target, as being a specialized host defense cell with well-known contribution to pathogenesis. Its surface contains receptors to recognize terminal galactose, mannose, fructose, or glucose residues of glycosides. Therefore, sugar-bearing liposomes are designed for improvement of new drugs to target the macrophage ,. At first, herbal medicine was not considered for development as novel formulations due to the lack of scientific justification and processing difficulties. However, NPs proved their use to deliver herbal medicine because of their unique physicochemical properties, their size and capacity to penetrate into the cell, as well as their ability to increase the therapeutic value by reducing toxicity and increasing bioavailability. That is besides its lower cost using natural herbs .
A microchip is a micro-fabricated device that includes a pump, valve, and flow channel to allow controlled release of single or multiple drugs on demand. It is implanted in patients who require pulsatile drug release. The drug reservoirs are covered by membranes formed of PLGA . Carbon nanotubes consist of graphite sheets rolled up into tubular forms. They can cross the cell membrane without membrane disruption to be localized into the cytosol and mitochondria. They are manufactured for drug delivery and gene therapy . Moreover, most of the new drug targets that emerged from high-throughput drug screening initiatives are insoluble or poorly soluble in water. Therefore, the therapeutic agents are conjugated or encapsulated or adsorbed on NPs or liposomes or nanospheres to overcome the drug solubility issue ,.
As sole treatment, targeting infected macrophages with NPs is a valuable and validated strategy for treatment of VL . Combined therapy by silver, CS, and curcumin NPs gave the highest effect and complete cure in giardiasis in experimentally infected animals . Silver and CS were evaluated singly or combined for in vivo treatment of toxoplasmosis in experimental animals. The combined treatment showed significant decrease in hepatic and splenic parasite burden. Microscopic examination revealed stoppage of movement and deformity in the shape of tachyzoites .
As a drug delivery system, quercetin conjugated with gold NPs was established for treatment of VL caused by wild-type resistant strains . On the other hand, CS proved to be a suitable drug delivery mean for several drugs used in VL treatment. Doxorubicin displayed significant reduction in Leishmania amastigotes (in vivo) and promastigotes (in vitro) , while amphotericin B  and rifampicin  gave significant results compared with control drugs without CS. Amphotericin B was also encapsulated in PLGA NPs and gave significantly effective results in comparison with the drug alone . A review article discussed all the studies that employed nanotechnology in drug delivery systems for amphotericin B in the treatment of VL. The reviewers concluded that CS and chondroitin sulfate NPs are the best ones nowadays due to their lower costs . In cutaneous leishmaniasis, glucantime formulated with liposomes was effective in the topical treatment of leishmanial ulcers caused by L. major in mice. It resulted in a significant decrease in lesion size and spleen parasite burden .
Isolated fungus from the soil (Trichoderma harzianum) conjugated with silver NPs increased the efficacy of triclabendazole in the treatment of fascioliasis . Binding of curcumin  and choloroquie  to CS increased chemical stability and enhanced bioavailability when evaluated in the treatment of malaria in infected mice. In cryptosporidiosis, polyvinyl alcohol conjugated with CS was proven to suppress the attachment of Cryptosporidium sporozoites to enterocytes in vitro . Albendazole bound to CS was effective in the treatment of alveolar echinococciosis caused by E. multilocularis  and visceral larva migrans caused by T. canis . In the first report, it was concluded that CS improved albendazole absorption, increased its bioavailability in vivo, is easily manufactured as capsules or tablets, and above all is nontoxic and of low cost. In the treatment of schistosomiasis mansoni, praziquantel (300 mg/kg) encapsulated in liposomes showed a significant reduction in worm burden and stool and intestinal egg counts as well as in the number of hepatic granulomas . Diminazene aceturate also encapsulated into liposomes, showed in vitro and in vivo significant results in treatment of Suda, caused by T. evans . Recently, miltefosine, an anticancer therapy, was enclosed in lipid nanocapsules and administered to S. mansoni-infected mice as a single oral dose (20 mg/kg), and its efficacy was compared with that of praziquantel. The results proved its potentiality in schistosomiasis mansoni, and the ability of nanomedicine to act as efficient drug delivery vehicle .
Drug delivery system in herbal medicine: For arthropods, an aqueous extract of neem leaves conjugated with silver NPs proved to be a potent insecticide against mosquitoes larvae, pupas, and adults for control of vector-transmitted diseases . Recently, several publications utilized synthesized silver NPs with different herbs (Barleriacristata spp., Aristolochiaindica spp., and Pteridiumaquilinum spp.) for testing against larvae of different mosquitoes (species of Anopheles, Aedes and Culex), and they showed significant larvicidal effects compared with using herbs alone ,,,. For parasitic diseases, essential oil of Achyrocline satureioides was given in an ananoencapsulated formula to experimentally T. evansi-infected mice and its efficacy was investigated in comparison with diminazeneaceturate and free essential oil. The encapsulated form showed better anti-trypanosomal activities without hepatic or renal toxicity, i.e. the use of nanotechnology reduced the side effects of the therapeutic agents . Synthesized palladium NPs using Eclipta prostrata leaf aqueous extract  and synthesized silver NPs using Euphorbia prostrata leaf extracts  or Acacia auriculiformis extracts  were tested against P. berghei, L. donovani, and the bovine filarial parasite Setaria cervi, respectively. The results showed the efficacy of NPs as potent drug delivery systems in herbal medicine manufacturing.
Immunization and vaccination
With variable effects, several NPs were formulated to optimize vaccine development as they protect DNA vaccines from degradation, and the use of chemical components (SAP, SL, PAMAM, PLGA, γ-PGA, and ARC) is considered a hopeful approach in vaccine development .
As prophylactic candidate: Since SAP NPs have the ability to induce CD8+ and CD4+T cells, they were used to stimulate long-lasting immune responses to specific epitope of P. falciparum circumsporozoite protein , and to vaccinate mice against toxoplasmosis .
As an adjuvant: To improve the immune response in schistosomiasis, calcium NPs were used with anti-idiotypic antibody (NP30) to enhance cellular and humoral immune response , while CS was given with gene encoding S. japonicum ferritin . In VL, cationic SL NPs were used with a tri-fusion gene of L. tarentolae (lizard species nonpathogenic to humans) in a DNA vaccine. Genes encoding A2 antigen and cysteine proteinases (CPA and CPB) were used and yielded promising results . Immunoliposomes when given with soluble Leishmania antigen (SLA) improved immunization . In Chagas disease, ARC act as strong adjuvants when given with soluble T. cruzi antigens .
As delivery system: Human P. falciparum Pfs25 specific antibodies block parasite infectivity to mosquitoes, but the extent of blocking was insufficient for an effective transmission blocking vaccine. Recombinant Pfs25H was then conjugated with Pseudomonas aeruginosa exoprotein A and formulated in gel NPs to improve blockage of infectivity to mosquitoes. This blocking vaccine is in phase 1 human trial in USA . In schistosomiasis, S. mansoni antigen (Rho) is involved in a number of cell signaling pathways with effects on actin cytoskeleton, gene transcription, and membrane trafficking. It was conjugated with CS NPs containing Rho and CpG (as an adjuvant to induce interleukin-12) and produced moderate protection, with a reduction in hepatic granulomas . Lysine-modified PAMAM was used as a novel delivery system for S. japonicum DNA vaccine . In leishmaniasis, PLGA NPs ,, alginate microspheres , SL NPs , and cationic liposomes , were used as delivery systems with several antigens such as leishmanial cysteine proteinase I, L. major antigen, soluble Leishmania antigen, and Leishmania kinetoplasmid membrane protein-11 to induce protection against VL. In malaria, several trials were reported; PLGA NPs were used as a delivery system for P. vivax sporozoites antigen (VMP001) , whereas merozoite surface protein-1 was conjugated with iron oxide NPs  or combined NPs of PE and γ-PGA  or anionic NPs ,, or quantum dots NPs .
| Concluding remarks|| |
- In the last decade, the use of NPs received considerable interest because of their defined properties, and they were used in the development of diagnostic methods, therapeutic targets, and in protection and vaccination of tropical parasitic diseases.
- AFM and scanning tunneling are two main instruments developed to manipulate nanostructures and to use NPs in the early diagnosis of fatal parasitic diseases such as malaria and VL.
- Targeting infected macrophages with NPs is a valuable and validated strategy for treatment.
- NPs are novel drug delivery systems for herbal medicine to be used as potent insecticides against mosquitos’ different stages, i.e. new strategy in the control of vector-transmitted diseases.
- Silver and CS NPs are considered potent therapies in the treatment of toxoplasmosis and giardiasis.
- Metal NPs, CS, and liposomes are conjugated with several drugs, e.g. praziquantel, choloroquie, amphotericin B, rifampicin, and albendazole in the treatment of schistosomiasis, malaria, VL, and visceral larva migrans.
- NPs act as vaccine candidates against toxoplasmosis and malaria, as an adjuvant to improve immune response against schistosomiasis, VL and Chagas disease, and as a vaccine delivery system against malaria, schistosomiasis, and VL.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Poole CP (Ed.). Introduction to nanotechnology. Hoboken, NJ: John Wiley & Sons; 2003.
Moghimi SM, Hunter AC, Murray JC. Nanomedicine: current status and future prospects. FASEB J 2005; 19:311–330.
Lin PC, Lin S, Wang PC, Sridhar R. Techniques for physicochemical characterization of nanomaterials. Biotechnol Adv 2014; 32:711–726.
Hardman R. A toxicologic review of quantum dots: toxicity depends on physicochemical and environmental factors. Environ Health Perspect 2006; 114:165–172.
Said DE, Elsamad LM, Gohar YM. Validity of silver, chitosan, and curcumin nanoparticles as anti-Giardia
agents. Parasitol Res 2012; 111:545–554.
Gherbawy YA, Shalaby IM, El-Sadek MS, Elhariry HM, Abdelilah BA. The anti-fasciolasis properties of silver nanoparticles produced by Trichoderma harzianum
and their improvement of the anti-fasciolasis drug triclabendazole. Int J Mol Sci 2013; 14:21887–21898.
Gaafar MR, Mady RF, Diab RG, Shalaby TI. Chitosan and silver nanoparticles: promising anti-toxoplasma agents. Exp Parasitol 2014; 143:30–38.
Soni N, Prakash S. Silver nanoparticles: a possibility for malarial and filarial vector control technology. Parasitol Res 2014; 113:4015–4022.
Murugan K, Labeeba MA, Panneerselvam C, Dinesh D, Suresh U, Subramaniam J et al. Aristolochia indica
green-synthesized silver nanoparticles: a sustainable control tool against the malaria vector Anopheles stephensi
? Res Vet Sci 2015; 102:127–135.
Zahir AA, Chauhan IS, Bagavan A, Kamaraj C, Elango G, Shankar J et al.
Green synthesis of silver and titanium dioxide nanoparticles using Euphorbia prostrata
extract shows shift from apoptosis to G0/G1 arrest followed by necrotic cell death in Leishmania donovani
. Antimicrob Agents Chemother 2015; 59:4782–4799.
Govindarajan M, Benelli G. Facile biosynthesis of silver nanoparticles using Barleria cristata
: mosquitocidal potential and biotoxicity on three non-target aquatic organisms. Parasitol Res 2016; 115:925–935.
Govindarajan M, Rajeswary M, Veerakumar K, Muthukumaran U, Hoti SL, Benelli G. Green synthesis and characterization of silver nanoparticles fabricated using Anisomeles indica
: mosquitocidal potential against malaria, dengue and Japanese encephalitis vectors. Exp Parasitol 2016; 161:40–47.
Panneerselvam C, Murugan K, Roni M, Aziz AT, Suresh U, Rajaganesh R et al.
Fern-synthesized nanoparticles in the fight against malaria: LC/MS analysis of Pteridium aquilinum
leaf extract and biosynthesis of silver nanoparticles with high mosquitocidal and antiplasmodial activity. Parasitol Res 2016; 115:997–1013.
Saini P, Saha SK, Roy P, Chowdhury P, Sinha Babu SP. Evidence of reactive oxygen species (ROS) mediated apoptosis in Setariacervi
induced by green silver nanoparticles from Acacia auriculiformis
at a very low dose. Exp Parasitol 2016; 160:39–48.
Wang H, Lei C, Li J, Wu Z, Shen G, Yu R. A piezoelectric immunoagglutination assay for Toxoplasma gondii
antibodies using gold nanoparticles. Biosens Bioelectron 2004; 19:701–709.
Pissuwan D, Valenzuela SM, Miller CM, Cortie MB. A golden bullet? Selective targeting of Toxoplasma gondii
tachyzoites using antibody-functionalized gold nanorods. Nano Lett, 2007; 7:3808–3812.
Javier DJ, Castellanos-Gonzalez A, Weigum SE, White AC Jr, Richards-Kortum R. Oligonucleotide-gold nanoparticle networks for detection of Cryptosporidium parvum
heat shock protein 70 mRNA. J Clin Microbiol 2009; 47:4060–4066.
Guirgis BS, Sá e Cunha C, Gomes I, Cavadas M, Silva I, Doria G et al.
Gold nanoparticle-based fluorescence immunoassay for malaria antigen detection. Anal Bioanal Chem 2012; 402:1019–1027.
Das S, Roy P, Mondal S, Bera T, Mukherjee A. One pot synthesis of gold nanoparticles and application in chemotherapy of wild and resistant type visceral leishmaniasis. Colloids Surf B Biointerfaces 2013; 107:27–34.
Jeon W, Lee S, Manjunatha DH, Ban C. A colorimetric aptasensor for the diagnosis of malaria based on cationic polymers and gold nanoparticles. Anal Biochem 2013; 439:11–16.
Weigum SE, Castellanos-Gonzalez A, White AC Jr, Richards-Kortum R. Amplification-free detection of Cryptosporidium parvum
nucleic acids with the use of DNA/RNA-directed gold nanoparticle assemblies. J Parasitol 2013; 99:923–926.
Andreadou M, Liandris E, Gazouli M, Taka S, Antoniou M, Theodoropoulos G et al.
A novel non-amplification assay for the detection of Leishmania
spp. in clinical samples using gold nanoparticles. J Microbiol Methods 2014; 96:56–61.
Wu J, Peng Y, Liu X, Li W, Tang S. Evaluation of Wondfo rapid diagnostic kit (Pf-HRP2/PAN-pLDH) for diagnosis of malaria by using nano-gold immunochromatographic assay. Acta Parasitol 2014; 59:267–271.
De la Escosura-Muñiz A, Baptista-Pires L, Serrano L, Altet L, Francino O, Sánchez A et al.
Magnetic bead/gold nanoparticle double-labeled primers for electrochemical detection of isothermal amplified Leishmania
DNA. Small 2016; 12:205–213.
Feng ZQ, Zhong SG, Li YH, Li YQ, Qiu ZN, Wang ZM et al.
Nanoparticles as a vaccine adjuvant of anti-idiotypic antibody against schistosomiasis. Chin Med J (Engl) 2004; 117:83–87.
Pusic K, Aguilar Z, McLoughlin J, Kobuch S, Xu H, Tsang M et al.
Iron oxide nanoparticles as a clinically acceptable delivery platform for a recombinant blood-stage human malaria vaccine. FASEB J 2013; 27:1153–1166.
Hemadi A, Ekrami A, Oormazdi H, Meamar AR, Akhlaghi L, Samarbaf-Zadeh AR et al.
Bioconjugated fluorescent silica nanoparticles for the rapid detection of Entamoeba histolytica
. Acta Trop 2015; 145:26–30.
Kunjachan S, Gupta S, Dwivedi AK, Dube A, Chourasia MK. Chitosan-based macrophage-mediated drug targeting for the treatment of experimental visceral leishmaniasis. J Microencapsul 2011; 28:301–310.
Akhtar F, Rizvi MM, Kar SK. Oral delivery of curcumin bound to chitosan nanoparticles cured Plasmodium yoelii
infected mice. Biotechnol Adv 2012; 30:310–320.
Luzardo Álvarez A, Blanco García E, Guerrero Callejas F, Gómez Couso H, Blanco Méndez J. In vitro
evaluation of the suppressive effect of chitosan/polyvinyl alcohol microspheres on attachment of C. parvum
to enterocytic cells. Eur J Pharm Sci 2012; 47:215–227.
Oliveira CR, Rezende CM, Silva MR, Pêgo AP, Borges O, Goes AM. A new strategy based on SmRho protein loaded chitosan nanoparticles as a candidate oral vaccine against schistosomiasis. PLoS Negl Trop Dis 2012; 6:e1894.
Asthana S, Jaiswal AK, Gupta PK, Pawar VK, Dube A, Chourasia MK. Immunoadjuvant chemotherapy of visceral leishmaniasis in hamsters using amphotericin B-encapsulated nanoemulsion template-based chitosan nanocapsules. Antimicrob Agents Chemother 2013; 57:1714–1722.
Tripathy S, Mahapatra SK, Chattopadhyay S, Das S, Dash SK, Majumder S et al.
A novel chitosan based antimalarial drug delivery against Plasmodium berghei
infection. Acta Trop 2013; 128:494–503.
Chaubey P, Mishra B. Mannose-conjugated chitosan nanoparticles loaded with rifampicin for the treatment of visceral leishmaniasis. Carbohydr Polym 2014; 101:1101–1108.
Abulaihaiti M, Wu XW, Qiao L, Lv HL, Zhang HW, Aduwayi N et al.
Efficacy of albendazole-chitosan microsphere-based treatment for alveolar echinococcosis in mice. PLoS Negl Trop Dis 2015; 9:e0003950.
Shukla S, Arora V, Jadaun A, Kumar J, Singh N, Jain VK. Magnetic removal of entamoeba cysts from water using chitosan oligosaccharide-coated iron oxide nanoparticles. Int J Nanomedicine 2015; 10:4901–4917.
Heravi Shargh V, Jaafari MR, Khamesipour A, Jalali SA, Firouzmand H, Abbasi A et al.
Cationic liposomes containing soluble Leishmania
antigens (SLA) plus CpG ODNs induce protection against murine model of leishmaniasis. Parasitol Res 2012; 111:105–114.
Firouzmand H, Badiee A, Khamesipour A, Heravi Shargh V, Alavizadeh SH, Abbasi A et al.
Induction of protection against leishmaniasis in susceptible BALB/c mice using simple DOTAP cationic nanoliposomes containing soluble Leishmania
antigen (SLA). Acta Trop 2013; 128:528–535.
Frezza TF, Gremião MP, Zanotti-Magalhães EM, Magalhães LA, de Souza AL, Allegretti SM. Liposomal-praziquantel: efficacy against Schistosoma mansoni
in a preclinical assay. Acta Trop 2013; 128:70–75.
Kalat SA, Khamesipour A, Bavarsad N, Fallah M, Khashayarmanesh Z, Feizi E et al.
Use of topical liposomes containing meglumineantimoniate (glucantime) for the treatment of L. major
lesion in BALB/c mice. Exp Parasitol 2014; 143:5–10.
Eskandari F, Talesh GA, Parooie M, Jaafari MR, Khamesipour A, Saberi Z et al.
Immunoliposomes containing soluble Leishmania
antigens (SLA) as a novel antigen delivery system in murine model of leishmaniasis. Exp Parasitol 2014; 146:78–86.
Oliveira CB, Rigo LA, Rosa LD, Gressler LT, Zimmermann CE, Ourique AF et al.
Liposomes produced by reverse phase evaporation: in vitro
and in vivo
efficacy of diminazene aceturate against Trypanosoma evansi
. Parasitology. 2014; 141:761–769.
Panyam J, Labhasetwar V. Biodegradable nanoparticles for drug and gene delivery to cells and tissues. Adv Drug Deliv Rev 2004; 55:329–347.
Barrera MG, Leonardi D, Bolmaro RE, Echenique CG, Olivieri AC, Salomon CJ et al. In vivo
evaluation of albendazole microspheres for the treatment of Toxocara canis
larva migrans. Eur J Pharm Biopharm 2010; 75:451–454.
Tafaghodi M, Eskandari M, Kharazizadeh M, Khamesipour A, Jaafari MR. Immunization against leishmaniasis by PLGA nanospheres loaded with an experimental autoclaved Leishmania major (ALM) and Quillaja saponins. Trop Biomed 2010; 27:639–650.
Tafaghodi M, Eskandari M, Khamesipour A, Jaafari MR. Alginate microspheres encapsulated with autoclaved Leishmania major
(ALM) and CpG-ODN induced partial protection and enhanced immune response against murine model of leishmaniasis. Exp Parasitol 2011; 129:107–114.
Doroud D, Zahedifard F, Vatanara A, Najafabadi AR, Rafati S. Cysteine proteinase type I, encapsulated in solid lipid nanoparticles induces substantial protection against Leishmania major
infection in C57BL/6 mice. Parasite Immunol 2011; 33:335–348.
Moon JJ, Suh H, Polhemus ME, Ockenhouse CF, Yadava A, Irvine DJ. Antigen-displaying lipid-enveloped PLGA nanoparticles as delivery agents for a Plasmodium vivax
malaria vaccine. PLoS One 2012; 7:e 31472.
Santos DM, Carneiro MW, de Moura TR, Fukutani K, Clarencio J, Soto M et al.
Towards development of novel immunization strategies against leishmaniasis using PLGA nanoparticles loaded with kinetoplastid membrane protein-11. Int J Nanomedicine 2012; 7:2115–2127.
Wang X, Dai Y, Zhao S, Tang J, Li H, Xing Y et al.
PAMAM-Lys, a novel vaccine delivery vector, enhances the protective effects of the SjC23 DNA vaccine against Schistosoma japonicum
infection. PLoS One 2014; 9:e86578.
Higa LH, Corral RS, Morilla MJ, Romero EL, Petray PB. Archaeosomes display immunoadjuvant potential for a vaccine against Chagas disease. Hum Vaccin Immunother 2013; 9:409–412.
Cherif MS, Shuaibu MN, Kodama Y, Kurosaki T, Helegbe GK, Kikuchi M et al.
Nanoparticle formulation enhanced protective immunity provoked by PYGPI8p-transamidase related protein (PyTAM) DNA vaccine in Plasmodium yoelii
malaria model. Vaccine 2014; 32:1998–2006.
Thiramanas R, Jangpatarapongsa K, Asawapirom U, Tangboriboonrat P, Polpanich D. Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection. Microb Biotechnol 2013; 6:406–413.
Cheung YW, Kwok J, Law AW, Watt RM, Kotaka M, Tanner JA. Structural basis for discriminatory recognition of Plasmodium lactate dehydrogenase by a DNA aptamer. Proc Natl Acad Sci USA 2013; 110:15967–15972.
Yuen C, Liu Q. Optimization of Fe3O4@Ag nanoshells in magnetic field-enriched surface-enhanced resonance Raman scattering for malaria diagnosis. Analyst 2013; 138:6494–6500.
Lukianova-Hleb EY, Campbell KM, Constantinou PE, Braam J, Olson JS, Ware RE et al.
Hemozoin-generated vapor nanobubbles for transdermal reagent- and needle-free detection of malaria. Proc Natl Acad Sci USA 2014; 111:900–905.
Hegazy S, Farid A, Rabae I, El-Amir A. Novel IMB-ELISA assay for rapid diagnosis of human toxoplasmosis using SAG1 antigen. Jpn J Infect Dis 2015; 68:474–480.
Owais M, Gupta CM. Targeted drug delivery to macrophages in parasitic infections. Curr Drug Deliv 2005; 2:311–318.
Devi VK, Jain N, Valli KS. Importance of novel drug delivery systems in herbal medicines. Pharmacogn Rev 2010; 4:27–31.
Grayson ACR, Choi IS, Tyler BM, Wang PP, Brem H, Cima MJ et al.
Multi-pulse drug delivery from a resorable polymeric microchip device. Nat Mat 2003; 2:767–772.
Bianco A. Carbon nanotubes for the delivery of therapeutic molecules. Expert Opin Drug Deliv 2004; 1:57–65.
Allen TM, Cullis PR. Drug delivery systems: entering the mainstream. Science 2004; 303:1818–1822.
Kunjachan S, Jose S, Thomas CA, Joseph E, Kiessling F, Lammers T. Physicochemical and biological aspects of macrophage-mediated drug targeting in anti-microbial therapy. Fundam Clin Pharmacol 2012; 26:63–71.
Kumar R, Sahoo GC, Pandey K, Das V, Das P. Study the effects of PLGA-PEG encapsulated amphotericin B nanoparticle drug delivery system against Leishmania donovani
. Drug Deliv 2015; 22:383–388.
Chávez-Fumagalli MA, Ribeiro TG, Castilho RO, Fernandes SO, Cardoso VN, Coelho CS et al.
New delivery systems for amphotericin B applied to the improvement of leishmaniasis treatment. Rev Soc Bras Med Trop 2015; 48:235–242.
El-Moslemany RM, Eissa MM, Ramadan AA, El-Khordagui LK, El-Azzouni MZ. Miltefosine lipid nanocapsules: intersection of drug repurposing and nanotechnology for single dose oral treatment of pre-patent schistosomiasis mansoni
. Acta Trop 2016; 159:142–148.
Do Carmo GM, Baldissera MD, Vaucher RA, Rech VC, Oliveira CB, Sagrillo MR et al.
Effect of the treatment with Achyrocline satureioides
(free and nanocapsules essential oil) and diminazene aceturate on hematological and biochemical parameters in rats infected by Trypanosoma evansi
. Exp Parasitol 2015; 149:39–46.
Rajakumar G, Rahuman AA, Chung IM, Kirthi AV, Marimuthu S, Anbarasan K. Antiplasmodial activity of eco-friendly synthesized palladium nanoparticles using Eclipta prostrata
extract against Plasmodium berghei
in Swiss albino mice. Parasitol Res 2015; 114:1397–1406.
Fahmy TM, Demento SL, Caplan MJ, Mellman I, Saltzman WM. Design opportunities for actively targeted nanoparticle vaccines. Nanomedicine (Lond) 2008; 3:343–355.
Kaba SA, McCoy ME, Doll TA, Brando C, Guo Q, Dasgupta D et al.
Protective antibody and CD8+
T-cell responses to the Plasmodium falciparum
circumsporozoite protein induced by a nanoparticle vaccine. PLoS One 2012; 7:e48304.
El Bissati K, Zhou Y, Dasgupta D, Cobb D, Dubey JP, Burkhard P et al.
Effectiveness of a novel immunogenic nanoparticle platform for Toxoplasma
peptide vaccine in HLA transgenic mice. Vaccine 2014; 32:3243–3248.
Chen LY, Yi XY, Zeng XF, Zhang SK, McReynolds L. Mucosal immunization of recombinant Schistosoma japonicum
ferritin. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 2004; 22:129–132.
Saljoughian N, Taheri T, Zahedifard F, Taslimi Y, Doustdari F, Bolhassani A et al.
Development of novel prime-boost strategies based on a tri-gene fusion recombinant L. tarentolae
vaccine against experimental murine visceral leishmaniasis. PLoS Negl Trop Dis 2013; 7:e 2174.
Shimp RL Jr, Rowe C, Reiter K, Chen B, Nguyen V, Aebig J et al.
Development of a Pfs25-EPA malaria transmission blocking vaccine as a chemically conjugated nanoparticle. Vaccine 2013; 31:2954–2962.
Cherif MS, Shuaibu MN, Kurosaki T, Helegbe GK, Kikuchi M, Yanagi T et al.
Immunogenicity of novel nanoparticle-coated MSP-1 C-terminus malaria DNA vaccine using different routes of administration. Vaccine 2011; 29:9038–9050.
Shuaibu MN, Cherif MS, Kurosaki T, Helegbe GK, Kikuchi M, Yanagi T et al.
Effect of nanoparticle coating on the immunogenicity of plasmid DNA vaccine encoding P. yoelii
MSP-1 C-terminal. Vaccine 2011; 29:3239–3247.
Pusic K, Xu H, Stridiron A, Aguilar Z, Wang A, Hui G. Blood stage merozoite surface protein conjugated to nanoparticles induce potent parasite inhibitory antibodies. Vaccine 2011; 29:8898–8908.
|This article has been cited by|
||Evaluation of nanoselenium and nanogold activities against murine intestinal schistosomiasis
| ||Mohamed A. Dkhil,Mona F. Khalil,Marwa S.M. Diab,Amira A. Bauomy,Simeon Santourlidis,Esam M. Al-Shaebi,Saleh Al-Quraishy |
| ||Saudi Journal of Biological Sciences. 2018; |
|[Pubmed] | [DOI]|
||Biosynthesized colloidal silver and gold nanoparticles as emerging leishmanicidal agents: an insight
| ||Muhammad Ovais,Akhtar Nadhman,Ali Talha Khalil,Abida Raza,Fazli Khuda,Muhammad Farhan Sohail,Nazar Ul Islam,Hafiz Shoaib Sarwar,Gul Shahnaz,Irshad Ahmad,Muthupandian Saravanan,Zabta Khan Shinwari |
| ||Nanomedicine. 2017; 12(24): 2807 |
|[Pubmed] | [DOI]|